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WebStack Overflow The World’s Largest Online Community for Developers Web5 de jul. de 2003 · Airline: Virgin Blue Airlines Reg: VH-VBC photos Aircraft: Boeing 737-7Q8 Serial #: 30638 Photo date: 2001-11-28 Uploaded: 2024-07-13 Likes: 1 0 Views: 1,162 Location: Sydney Kingsford Smith Int'l Airport - YSSY, Australia By: PAUL LINK Photos Profile Contact Album Like Share Airline: Virgin Blue Airlines Reg: VH-VBC photos Human and mouse genome-wide sgRNA libraries were designed to target 18,659 and 19,248 RefSeq genes, respectively, with six sgRNAs per gene. To select sgRNAs that lead to full protein loss on frameshift mutation, we identified all PAM sites (NGG) on both strands in the first 1 kilobases of the longest coding … Ver mais The human and mouse sgRNA library pools were printed on two 244K arrays (Agilent Technologies) as a total of 27 subpools each defined by a biological category of genes. The focused sgRNA library used for … Ver mais For pooled negative-selection screens, doxycycline-inducible Cas9 clones were generated for the human cell lines RKO, MiaPaca and KBM7. First, cells were sequentially transduced with pWPXLd-EF1A-rtTA3-IRES … Ver mais Human CRISPR library virus was produced by standard transient transfection of Lenti-X (Clontech, 632180) cells. Briefly, per 10-cm dish, 1 × 107 cells were seeded in the morning and transfected 8 h later. … Ver mais KBM-7 cells were routinely maintained in IMDM medium, RKO in RPMI, MiaPaca and Lenti-X in DMEM supplemented with 10% FCS, 1 mM sodium pyruvate, 4 mM l-glutamine and … Ver mais hry online formule