WebJan 1, 2014 · The metabolically quenched cell pellets (single sample, 4 × 10 7 parasites total) are resuspended in pre-chilled PBS (1 ml) and transferred to 1.5 ml safe-lock … WebAug 17, 2024 · The NR viability assay kit (Suzhou Kemin Biological Co., Ltd., item no. NR-2-Y) was used for NR viability determination. One hundred milligrams of material was homogenized by grinding in 1 mL of pre-chilled PBS (50 mmol/L, pH 7.0) buffer in an ice bath and centrifuged at 8000 rpm for 10 min at 4℃.
RIPA Lysis Buffer - Boster Bio
WebJun 25, 2024 · The dried lipids were hydrated with 250 µl pre-chilled PBS buffer in the range of 2–10 mg/ml and sonicated in a water bath for 30 min, maintaining a temperature of ~ 4 °C. WebSep 6, 2024 · The cells were then spinned down at 2000 rpm for 5 min and resuspended in pre-chilled PBS buffer at 2 × 10 5 cells/mL density. Cells were stained with Armenian hamster anti-CD29 (1:500 ... hammondindustriesinc.com
Pre Chilled Pbs Thermo Fisher Bioz
WebJul 19, 2024 · 1. Cut 50-100 mg of tissues into small pieces, wash minced tissues with 1 ml of pre-chilled PBS and centrifuge at 500×g for 3 minutes, gently discard the supernatant. Repeat the wash once. 2. Add 1 ml of CPEB I to tissues and mix thoroughly by vortexing. Transfer the suspension to a pre-chilled glass homogenizer and U87, T98, LN229, U251 and A172, were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA), HA1800 were purchased from Shanghai Honsun Biological Technology Co., Ltd (Shanghai, China). See more TMZ dissolved in DMSO, working concentration is 1μmol/L, was obtained from Sigma (Cat. T2577-25MG), DMSO was obtained from … See more Six human glioma cell lines, including U87, T98, LN229, U251, A172 and HA1800 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum … See more Gliomablastoma and adjacent non-tumor tissues were obtained from the First Affiliated Hospital of Zhengzhou University (Zhengzhou, Henan, … See more Glioblastoma cells, U87 and U251 (3.0 × 105) stably expressing miR-128-3p were seeded on a 6-well plate and cultured to form a monolayer. … See more WebJul 24, 2015 · Testes were cleaned in pre-chilled PBS on ice and weighed using balance (CPA225D, Sartorius, Göttingen). Testes homogenates were prepared in pre-chilled PBS … hammond in newspaper hammond times