WebDec 22, 2024 · As described in this issue of Nature Chemical Biology, to create a fluorescent RNA with improved characteristics, Dey et al. 4 have repurposed a well-known adenine … WebSpinach riboswitches constitute a novel class of RNA-based fluo- rescent metabolite sensors that take advantage of the evolution- arily optimized affinity and selectivity seen …
PNAS
WebRiboswitch-based reporters can be constructed by replacing the downstream gene of a well-characterized riboswitch with a reporter gene such as LacZ for colorimetric measurement, GFP for fluorescence, or luciferase for luminescence. ... such as Spinach , so that ligand binding results in a conformational change that directly activates the ... WebTo gain a better understanding of the riboswitch regulatory mechanism, to allow for more efficient riboswitch optimization and use for biosensing applications, we studied the performance of a theophylline-responsive synthetic riboswitch coupled with the superfolder green fluorescent protein (sfGFP) reporter gene in E. coli cellular extract and ... seinfeld cast deaths george cost
Developing Fluorogenic Riboswitches for Imaging Metabolite ...
WebHere we describe Spinach riboswitches, a novel class of genetically encoded me- tabolite sensor based on riboswitches, a group of naturally oc- curring ligand-binding RNAs. Spinach riboswitches use Spinach, an RNA aptamer that binds and activates the fluorescence of an otherwise nonfluorescent small-molecule fluorophore. WebDec 12, 2024 · The adenine riboswitch was sensitive to Mg2+ concentrations and could be used as a biosensor to measure cellular Mg2+ concentrations. Additionally, the TQ3/Cy3-labeled adenine riboswitch yielded a Mg2+ concentration that was similar to that measured using a commercial assay kit. ... In recent years, spinach, mango, and broccoli aptamers … http://govform.org/broccoli-aptamer-lab-report seinfeld cast member who died